Objective: To investigate the correlation between sperm DNA fragmentation rate, sperm morphology rate and sperm acrosin and its notable in judging pregnancy outcome. Methods: Forty infertile couples admitted to the First Affiliated Hospital of Nanchang University from March 2020 to June 2022 were opted as the study cluster, and 40 healthy subjects with normal physical examination results in the same period were opted as the control cluster. Various parameters and indicators of semen were compared between the two clusters, and multivariate logistic regression analysis was performed according to the factors affecting the sperm DNA fragmentation rate. Pearson correlation analysis was performed between the sperm DNA fragmentation rate, sperm morphology rate and sperm acrosin, and the pregnancy outcomes under semen with variate sperm DNA fragmentation rates were compared.
Results: The semen concentration, normal sperm morphology rate, progressive motile sperm volume and sperm acrosin activity in the study cluster were bottom than those in the control cluster, and the sperm DFI index was upper than that in the control cluster (P < 0.05); Age, semen concentration, progressive motile sperm volume, normal sperm morphology rate, smoking, sperm acrosin activity and irregular life routines were independent factors affecting sperm DFI (≥10%) (P < 0.05); Sperm DFI was positively correlated with age and negatively correlated with other sperm DFI. Sperm activity was negatively correlated with age and positively correlated with other activities. The fertilization rate of sperm DFI < 10% group was higher than that of sperm DFI≥10% group (P < 0.05), but there were no significant differences in cleavage rate, excellent embryo rate and pregnancy rate (P > 0.05).
Conclusion: Smoking, irregular living habits and age will affect the sperm DNA fragmentation rate, and the DNA fragmentation rate will have a direct impact on the sperm morphology rate and the activity of sperm acrosin, while sperm acrosin will affect the normal sperm morphology rate. There is a certain correlation between the three, but it has little effect on pregnancy outcomes.
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Xu, Q., Ma, J., Yi, H., Huang, J. (2025) The Correlation between Sperm DNA Fragmentation Rate, Sperm Morphology Rate, and Sperm Acrosome Enzyme and Its Value for Predicting Pregnancy Outcome. Journal of Disease and Public Health, 1(1), 1-8.