The widespread use of antibiotics in biomedical fields has led to their substantial residues in the environment, and the resulting bacterial resistance has become a global health threat. Conventional antibiotic wastewater treatment processes have limited capacity for antibiotic removal. As an emerging bio electrochemical technology, microbial fuel cells (MFCs) can degrade antibiotics while simultaneously recovering electrical energy, demonstrating broad prospects in the field of antibiotic wastewater treatment. This review comprehensively introduces and summarizes the degradation performance and electricity generation of MFCs toward different types of antibiotics, the treatment of antibiotic wastewater by various biological processes, and discusses the advantages and challenges of coupled MFC systems in synergistically enhancing degradation, reducing antibiotic resistance genes, and improving electricity generation performance. This paper aims to provide a reference for in-depth research and practical application of MFCs and their coupled technologies in antibiotic wastewater treatment.
References
[1] Zhu, L., Lin, X., Di, Z., Cheng, F., Xu, J. (2024) Occurrence, risks, and removal methods of antibiotics in urban wastewater treatment systems: a review. Water, 16(23), 3428.
[2] Xu, R., Yang, Z. H., Zheng, Y., Wang, Q. P., Bai, Y., Liu, J. B., Fan, C. Z. (2019) Metagenomic analysis reveals the effects of long-term antibiotic pressure on sludge anaerobic digestion and antimicrobial resistance risk. Bioresource Technology, 282, 179-188.
[3] Zhang, Q. Q., Ying, G. G., Pan, C. G., Liu, Y. S., Zhao, J. L. (2015) Comprehensive evaluation of antibiotics emission and fate in the river basins of China: source analysis, multimedia modeling, and linkage to bacterial resistance. Environmental Science & Technology, 49(11), 6772-6782.
[4] Qiao, M., Ying, G. G., Singer, A. C., Zhu, Y. G. (2018) Review of antibiotic resistance in China and its environment. Environment International, 110, 160-172.
[5] Lorenzo, P., Adriana, A., Jessica, S., Carles, B., Marinella, F., Marta, L., Pierre, S. (2018) Antibiotic resistance in urban and hospital wastewaters and their impact on a receiving freshwater ecosystem. Chemosphere, 206, 70-82.
[6] Rizzo, L., Manaia, C., Merlin, C., Schwartz, T., Dagot, C., Ploy, M. C., Fatta-Kassinos, D. (2013) Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: a review. Science of the Total Environment, 447, 345-360
[7] Sabri, N. A., Van Holst, S., Schmitt, H., van der Zaan, B. M., Gerritsen, H. W., Rijnaarts, H. H., Langenhoff, A. A. M. (2020) Fate of antibiotics and antibiotic resistance genes during conventional and additional treatment technologies in wastewater treatment plants. Science of the Total Environment, 741, 140199.
[8] Liang, L., Lin, Y., Ren, R., Zhang, Q., Qu, J., Liu, Y., Sun, X. (2025) Recent advances in antibiotic removal using independent and coupled microbial fuel cells (MFCs): Mechanisms and cells (MFCs): Mechanisms and practical applications. Journal of Water Process Engineering, 71, 107226.
[9] Slate, A. J., Whitehead, K. A., Brownson, D. A., Banks, C. E. (2019) Microbial fuel cells: An overview of current technology. Renewable and Sustainable Energy Reviews, 101, 60-81.
[10] Yang, Z., ZHU, C., Tian, Y. (2020) Research Progresses in Microbial Fuel Cells for Antibiotic Wastewater Treatment. Chinese Journal of Agrometeorology, 41(05), 275.
[11] Esfandyari, M., Jafari, D., Azami, H. (2024) Microbial fuel cells for energy production in wastewater treatment plants-a review. Biofuels, 15(6), 743-753.
[12] Radeef, A. Y., Najim, A. A. (2024) Microbial fuel cell: The renewable and sustainable magical system for wastewater treatment and bioenergy recovery. Energy, 360, 1, 100001.
[13] Bazina, N., Ahmed, T. G., Almdaaf, M., Jibia, S., Sarker, M. (2023) Power generation from wastewater using microbial fuel cells: a review. Journal of Biotechnology, 374, 17-30.
[14] Muwakhidah, I. I., Sharma, M., Hassan, S. H. (2026) Bioelectricity and microbial fuel cells: principles, materials, and applications. Artificial Intelligence in Biofuels Production, 319-367.
[15] Nasrabadi, A. M. (2026) A detailed survey of microbial fuel cells: Classifications, computational modeling, recent innovations, and emerging applications. Renewable and Sustainable Energy Reviews, 226, 116241.
[16] Zhang, Z., Qiu, Y., Wang, Y., Yu, M., Ma, Z., Wang, R., Liu, S. (2026) Entropy‐Engineered HEO/Fe, N‐CNT bioanode via flash joule heating: accelerated electron harvesting and directed geobacter enrichment for high‐power microbial fuel vells. Small, 22(5), e11164.
[17] Liu, L., Lu, Y., Yuan, J., Zhu, H., Huang, S., Yang, B., Feng, Z. (2022) Effects of chloramphenicol on denitrification in single-chamber microbial fuel cell: comprehensive performance and bacterial community structure. Biochemical Engineering Journal, 182, 108429.
[18] Zhang, E., Yu, Q., Zhai, W., Wang, F., Scott, K. (2018) High tolerance of and removal of cefazolin sodium in single-chamber microbial fuel cells operation. Bioresource Technology, 249, 76-81.
[19] Mukhopadhyay, D., Khan, N., Kamal, N., Varjani, S., Singh, S., Sindhu, R., Bhargava, P. C. (2022) Degradation of β-lactam antibiotic ampicillin using sustainable microbial peroxide producing cell system. Bioresource Technology, 361, 127605.
[20] Gao, Y. X., Li, X., Fan, X. Y., Zhao, J. R., Zhang, Z. X. (2022) Wastewater treatment plants as reservoirs and sources for antibiotic resistance genes: a review on occurrence, transmission and removal. Journal of Water Process Engineering, 46, 102539.
[21] Cheng, P., Usman, M., Arslan, M., Sun, H., Zhou, L., Gamal El-Din, M. (2023) Enhancing biodegradation of pyridine with trehalose lipid in rhodococcus pyridinivorans sp. strain HR-1-inoculated microbial fuel cell. Fermentation, 9(2), 133.
[22] Yang, X. L., Wang, Q., Li, T., Xu, H., Song, H. L. (2022) Antibiotic removal and antibiotic resistance genes fate by regulating bioelectrochemical characteristics in microbial fuel cells. Bioresource Technology, 348, 126752.
[23] Ondon, B. S., Li, S., Zhou, Q., Li, F. (2020) Simultaneous removal and high tolerance of norfloxacin with electricity generation in microbial fuel cell and its antibiotic resistance genes quantification. Bioresource Technology, 304, 122984.
[24] Yan, W., Wang, S., Ding, R., Tian, X., Bai, R., Gang, H., Zhao, F. (2019) Long-term operation of electroactive biofilms for enhanced ciprofloxacin removal capacity and anti-shock capabilities. Bioresource Technology, 275, 192-199.
[25] Ghanam, A., Cecillon, S., Mohammadi, H., Amine, A., Buret, F., Haddour, N. (2023) Selective sensing in microbial fuel cell biosensors: insights from toxicity-adapted and non-adapted biofilms for Pb (II) and neomycin sulfate detection. Micromachines, 14(11), 2027.
[26] Jiang, J., Wang, H., Zhang, S., Li, S., Zeng, W., Li, F. (2021) The influence of external resistance on the performance of microbial fuel cell and the removal of sulfamethoxazole wastewater. Bioresource Technology, 336, 125308.
[27] Wen, H., Zhu, H., Xu, Y., Yan, B., Shutes, B., Bañuelos, G., Wang, X. (2021) Removal of sulfamethoxazole and tetracycline in constructed wetlands integrated with microbial fuel cells influenced by influent and operational conditions. Environmental Pollution, 272, 115988.
[28] Al-Ansari, M. M., Benabdelkamel, H., Al-Humaid, L. (2021) Degradation of sulfadiazine and electricity generation from wastewater using Bacillus subtilis EL06 integrated with an open circuit system. Chemosphere, 276, 130145.
[29] Yurdakok-Dikmen, B., Tresnakova, N., Filazi, A., Faggio, C. (2023) Biological wastewater treatment systems for the biodegradation and detoxification of pharmaceuticals. Pharmaceuticals in Aquatic Environments, 65-86.
[30] Cetecioglu, Z., Ince, B., Orhon, D., Ince, O. (2016) Anaerobic sulfamethoxazole degradation is driven by homoacetogenesis coupled with hydrogenotrophic methanogenesis. Water Research, 90, 79-89.
[31] Shi, X., Leong, K. Y., Ng, H. Y. (2017) Anaerobic treatment of pharmaceutical wastewater: a critical review. Bioresource Technology, 245, 1238-1244.
[32] Liu, H., Pu, C., Yu, X., Sun, Y., Chen, J. (2018) Removal of tetracyclines, sulfonamides, and quinolones by industrial-scale composting and anaerobic digestion processes. Environmental Science and Pollution Research, 25(36), 35835-35844.
[33] Yin, F., Dong, H., Zhang, W., Zhu, Z., Shang, B. (2018) Antibiotic degradation and microbial community structures during acidification and methanogenesis of swine manure containing chlortetracycline or oxytetracycline. Bioresource Technology, 250, 247-255.
[34] Huang, J., Wu, F., Xiao, Y., Ye, M., Wu, X., Chen, H., Xu, Q. (2025) Deciphering nitrogen-driven microbial succession in an anaerobic membrane bioreactor-coupled A2/O ecological system for the remediation of industrial swinewastewater. Scientific Reports, 15(1), 28422.
[35] Balcıoğlu, G., Vergili, I., Gönder, Z. B., Yilmaz, G. Ü. L. S. Ü. M., Bacaksiz, A. M., Kaya, Y. A. S. E. M. İ. N. (2023) Effect of powdered activated carbon addition on membrane performance and fouling in anaerobic membrane bioreactor. International Journal of Environmental Science and Technology, 20(3), 3191-3204.
[36] Oberoi, A. S., Jia, Y., Zhang, H., Khanal, S. K., Lu, H. (2019) Insights into the fate and removal of antibiotics in engineered biological treatment systems: a critical review. Environmental Science & Technology, 53(13), 7234-7264.
[37] Maddela, N. R., Abiodun, A. S., Zhang, S., Prasad, R. (2023) Biofouling in membrane bioreactors-mitigation and current status: a review. Applied Biochemistry and Biotechnology, 195(9), 5643-5668.
[38] Chen, J., Liu, Y. S., Zhang, J. N., Yang, Y. Q., Hu, L. X., Yang, Y. Y., Ying, G. G. (2017) Removal of antibiotics from piggery wastewater by biological aerated filter system: treatment efficiency and biodegradation kinetics. Bioresource Technology, 238, 70-77.
[39] Yan, W., Xiao, Y., Yan, W., Ding, R., Wang, S., Zhao, F. (2019) The effect of bioelectrochemical systems on antibiotics removal and antibiotic resistance genes: a review. Chemical Engineering Journal, 358, 1421-1437.
[40] Shang, W., Liu, Y., Cheng, D., Ngo, H. H., Guo, W., Liu, H., Liu, C. (2026) Antibiotic and Cu 2+ Co-selection in MFCs treating swine wastewater: antibiotic resistance genes dynamics and removal performance. Environmental Chemistry and Safety, 2(1), 9600008.
[41] Yan, L., Liang, B., Qi, M. Y., Wang, A. J., Liu, Z. P. (2022) Degrading characterization of the newly isolated Nocardioides sp. N39 for 3-amino-5-methyl-isoxazole and the related genomic information. Microorganisms, 10(8), 1496.
[42] Kong, D., Yun, H., Cui, D., Qi, M., Shao, C., Cui, D., Wang, A. (2017) Response of antimicrobial nitrofurazone-degrading biocathode communities to different cathode potentials. Bioresource Technology, 241, 951-958.
[43] Miran, W., Jang, J., Nawaz, M., Shahzad, A., Lee, D. S. (2018) Biodegradation of the sulfonamide antibiotic sulfamethoxazole by sulfamethoxazole acclimatized cultures in microbial fuel cells. Science of the Total Environment, 627, 1058-1065.
[44] Pan, J. J., Tan, L. Y., Fan, Q. Q., Cao, X. Y., Huang, J., Gu, Y. K., Chen, T. M. (2023) Effect of different carbon sources on sulfate reduction and microbial community structure in bioelectrochemical systems. Environmental Science and Pollution Research, 30(7), 18312-18324.
[45] Gebru, S. B., Werkneh, A. A. (2024) Applications of constructed wetlands in removing emerging micropollutants from wastewater: Occurrence, public health concerns, and removal performances-a review. South African Journal of Chemical Engineering, 48(1), 395-416.
[46] Fan, Z., Du, Y., Zhang, J., Yang, X. (2025) Pathogenic bacteria and high-risk ARGs removal in subsurface flow constructed wetlands treating swine tailwater: performance and influencing factors. Chemical Engineering Journal, 507, 160582.
[47] García, J., García-Galán, M. J., Day, J. W., Boopathy, R., White, J. R., Wallace, S., Hunter, R. G. (2020) A review of emerging organic contaminants (EOCs), antibiotic resistant bacteria (ARB), and antibiotic resistance genes (ARGs) in the environment: Increasing removal with wetlands and reducing environmental impacts. Bioresource Technology, 307, 123228.
[48] Gupta, S., Srivastava, P., Patil, S. A., Yadav, A. K. (2021) A comprehensive review on emerging constructed wetland coupled microbial fuel cell technology: Potential applications and challenges. Bioresource Technology, 320, 124376.
[49] Dai, M., Zhang, Y., Wu, Y., Sun, R., Zong, W., Kong, Q. (2021) Mechanism involved in the treatment of sulfamethoxazole in wastewater using a constructed wetland microbial fuel cell system. Journal of Environmental Chemical Engineering, 9(5), 106193.
[50] Li, H., Xu, H., Yang, Y. L., Yang, X. L., Wu, Y., Zhang, S., Song, H. L. (2019) Effects of graphite and Mn ore media on electro-active bacteria enrichment and fate of antibiotic and corresponding resistance gene in up flow microbial fuel cell constructed wetland. Water Research, 165, 114988.
[51] Sun, X., Zu, K., Liang, H., Sun, L., Zhang, L., Wang, C., Sharma, V. K. (2018) Electrochemical synthesis of ferrate (VI) using sponge iron anode and oxidative transformations of antibiotic and pesticide. Journal of Hazardous Materials, 344, 1155-1164.
[52] Sun, J., Li, N., Yang, P., Zhang, Y., Yuan, Y., Lu, X., Zhang, H. (2020) Simultaneous antibiotic degradation, nitrogen removal and power generation in a microalgae-bacteria powered biofuel cell designed for aquaculture wastewater treatment and energy recovery. International Journal of Hydrogen Energy, 45(18), 10871-10881.
[53] Li, S., Hua, T., Yuan, C. S., Li, B., Zhu, X., Li, F. (2020) Degradation pathways, microbial community and electricity properties analysis of antibiotic sulfamethoxazole by bio-electro-Fenton system. Bioresource Technology, 298, 122501.
[54] Li, S., Liu, Y., Ge, R., Yang, S., Zhai, Y., Hua, T., Li, F. (2020) Microbial electro-Fenton: A promising system for antibiotics resistance genes degradation and energy generation. Science of the Total Environment, 699, 134160.
[55] Liu, H., Yao, Y., Yuan, X., Hui, J., An, W., Xu, S., Zhang, Y. (2025) Advances and challenges in synergistic fenton-microbial fuel cell systems for emerging contaminants removal: mechanisms, configurations, and applications. Bioresource Technology, 133499.
[56] Liu, Q., Zhang, N., Ge, J., Zhang, L., Guo, L., Zhang, H., Yang, S. (2024) Aquatic plants combined with microbial fuel cells promote sulfamethoxazole and sul genes removal from aquaculture pond sediments via bioelectrochemistry. Environmental Pollution, 360, 124680.
[57] Wang, Y., Zhang, H., Feng, Y., Li, B., Yu, M., Xu, X., Cai, L. (2019) Bio-Electron-Fenton (BEF) process driven by sediment microbial fuel cells (SMFCs) for antibiotics desorption and degradation. Biosensors and Bioelectronics, 136, 8-15.
[58] Hu, X., Jiang, M. (2025) Photocatalytic microbial fuel cells for energy production and environmental remediation: a brief review. Journal of Environmental Chemical Engineering, 119905.
[59] Yu, T., Yang, B., Zhang, R., Yang, C., Jiang, J. (2024) Fabrication of a novel ZS-scheme photocatalytic fuel cell with the Z-scheme TiO2/GO/g-C3N4 photoanode and S-scheme BiOAc1−xBrx/BiOBr photocathode for TC degradation. Journal of Materials Science & Technology, 188, 11-26.
[60] Xu, P., Zheng, D., He, Q., Yu, J. (2020) The feasibility of ofloxacin degradation and electricity generation in photo-assisted microbial fuel cells with LiNbO3/CF photocatalytic cathode. Separation and Purification Technology, 250, 117106.
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Wei, Z., Hu, J. (2026) Research Progress on Microbial Fuel Cell Systems for Treating Antibiotic Wastewater. Journal of Disease and Public Health, 2(1), 35-44. https://doi.org/10.71052/jdph/VWPD1332