Enzymatic biofuel cells (EBFCs), as a novel type of green energy device, can directly convert biomass energy into electrical energy under mild conditions. They possess outstanding advantages such as good biocompatibility, environmental friendliness, and a wide range of raw material sources, demonstrating broad application prospects in fields such as wearable electronic devices, implantable medical devices, and environmental monitoring. Glucose oxidase (GOx), as the most commonly used anode biocatalyst in EBFCs, has become a research hotspot in this field due to its high specificity for glucose, high catalytic activity, and wide availability. This paper systematically reviews the research progress in the application of glucose oxidase in EBFCs, focusing on the catalytic mechanism of GOx, optimization of immobilization techniques, and its application effects in different types of EBFCs. It analyzes the core issues currently existing in the application of GOx in EBFCs, such as poor enzyme stability, low electron transfer efficiency, and short service life, and provides an outlook on its future development trends and application prospects. This offers important theoretical references and key technical insights for the subsequent development of high-performance GOx-based EBFCs.
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Zhu, H., Tang, S., Xiao, J., Ling, K., Wang, G., Yu, P., Li, Y., Jiang, Q., Lai, X., Wang, Z., Zou, S. (2026) Research Progress on the Application of Glucose Oxidase in Enzymatic Biofuel Cells. Scientific Research Bulletin, 3(1), 7-19. https://doi.org/10.71052/srb2024/BIQX4588