Nanozymes, as a class of nanomaterials with enzyme-like catalytic activity, combine the high catalytic efficiency of natural enzymes with the stability, ease of modification, and low cost of nanomaterials, making them a research hotspot in the field of electrochemical sensors. This article systematically reviews the classification, catalytic mechanisms, and performance regulation methods of nanozymes, focusing on the construction strategies and application progress of different types of nanozymes (metal-based, carbon-based, Metal-Organic Framework-based, etc.) in electrochemical sensors, covering multiple fields such as biomarker detection, food safety monitoring, and environmental pollutant analysis. Furthermore, the article analyzes the current bottlenecks in the practical applications of nanozyme-based electrochemical sensors, including insufficient catalytic selectivity, poor long-term stability, and difficulty in eliminating interference from actual samples. Finally, it provides an outlook on future development trends, aiming to offer reference and ideas for subsequent research in this field.
References
[1] Białas, K., Moschou, D., Marken, F., Estrela, P. (2022) Electrochemical sensors based on metal nanoparticles with biocatalytic activity. Microchimica Acta, 189(4), 172.
[2] Golchin, J., Golchin, K., Alidadian, N., Ghaderi, S., Eslamkhah, S., Eslamkhah, M., Akbarzadeh, A. (2017) Nanozyme applications in biology and medicine: an overview. Artificial Cells, Nanomedicine, and Biotechnology, 45(6), 1069-1076.
[3] Gao, L., Zhuang, J., Nie, L., Zhang, J., Zhang, Y., Gu, N., Wang, T., Feng, J., Yang, D., Perrett, S., Yan, X. (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nature Nanotechnology, 2(9), 577-583.
[4] Liang, M., Yan, X. (2019) Nanozymes: from new concepts, mechanisms, and standards to applications. Accounts of Chemical Research, 52(8), 2190-2200.
[5] Guo, G., Xu, S. H., Du, Y. T., Jiang, T. M., Song, J. L., Yang, Z. Q., Gao, Y. J. (2023) Potassium cobalt hexacyanoferrate as a peroxidase mimic for electrochemical immunosensing of Lactobacillus rhamnosus GG. Talanta, 264, 124746.
[6] Wang, Z., Wu, J., Zheng, J. J., Shen, X., Yan, L., Wei, H., Gao, X., Zhao, Y. (2021) Accelerated discovery of superoxide-dismutase nanozymes via high-throughput computational screening. Nature Communications, 12(1), 6866.
[7] Yang, Z., Guo, J., Wang, L., Zhang, J., Ding, L., Liu, H., Yu, X. (2024) Nanozyme‐enhanced electrochemical biosensors: mechanisms and applications. Small, 20(14), 2307815.
[8] Li, Y., Dong, Y., Wang, R., Lin, Z., Lin, J., Ji, X., Ye, B. C. (2024) Biomimetic electrochemical sensor based on single-atom nickel laccase nanoenzyme for quercetin detection. Analytical Chemistry, 96(6), 2610-2619.
[9] Tian, Q., Li, S., Tang, Z., Zhang, Z., Du, D., Zhang, X., Niu, X., Lin, Y. (2025) Nanozyme‐enabled biomedical diagnosis: advances, trends, and challenges. Advanced Healthcare Materials, 14(8), 2401630.
[10] Gonçalves, J. M., Martins, P. R., Rocha, D. P., Matias, T. A., Juliao, M. S., Munoz, R. A., Angnes, L. (2021) Recent trends and perspectives in electrochemical sensors based on MOF-derived materials. Journal of Materials Chemistry C, 9(28), 8718-8745.
[11] Zhang, L., Bi, X., Liu, X., He, Y., Li, L., You, T. (2023) Advances in the application of Metal-Organic Framework nanozymes in colorimetric sensing of heavy metal ions. Nanoscale, 15(31), 12853-12867.
[12] Hsu, C. Y., Alshik, N. M., Ahmad, I., Uthirapathy, S., Ballal, S., Singh, A., Saini, S., Joshi, K. K. (2025) Recent advances in MXene nanozyme-based optical and electrochemical biosensors for food safety analysis. Nanoscale, 17(13), 7697-7712.
[13] Liu, Q., Zhang, A., Wang, R., Zhang, Q., Cui, D. (2021) A review on metal-and metal oxide-based nanozymes: properties, mechanisms, and applications. Nano-Micro Letters, 13(1), 154.
[14] Demkiv, O., Stasyuk, N., Serkiz, R., Gayda, G., Nisnevitch, M., Gonchar, M. (2021) Peroxidase-like metal-based nanozymes: synthesis, catalytic properties, and analytical application. Applied Sciences, 11(2), 777.
[15] Kumar, V., Bano, D., Singh, D. K., Mohan, S., Singh, V. K., Hasan, S. H. (2018) Size-dependent synthesis of gold nanoparticles and their peroxidase-like activity for the colorimetric detection of glutathione from human blood serum. ACS Sustainable Chemistry & Engineering, 6(6), 7662-7675.
[16] Gao, M., Lu, X., Chen, S., Tian, D., Zhu, Y., Wang, C. (2018) Enhanced peroxidase-like activity of Mo6+-doped Co3O4 nanotubes for ultrasensitive and colorimetric L-cysteine detection. ACS Applied Nano Materials, 1(9), 4703-4715.
[17] Wei, C., Zou, X., Liu, Q., Li, S., Kang, C., Xiang, W. (2020) A highly sensitive non-enzymatic glucose sensor based on CuS nanosheets modified Cu2O/CuO nanowire arrays. Electrochimica Acta, 334, 135630.
[18] Li, F., Jiang, J., Peng, H., Li, C., Li, B., He, J. (2022) Platinum nanozyme catalyzed multichannel colorimetric sensor array for identification and detection of pesticides. Sensors and Actuators B: Chemical, 369, 132334.
[19] Shu, H., Chang, G., Su, J., Cao, L., Huang, Q., Zhang, Y., Xia, T., He, Y. (2015) Single-step electrochemical deposition of high performance Au-graphene nanocomposites for nonenzymatic glucose sensing. Sensors and Actuators B: Chemical, 220, 331-339.
[20] Arul, P., Nandhini, C., Huang, S. T., Huang, C. H., Gowthaman, N. S. K. (2025) Ni-MOF embedded with carbon nanomaterials/polypyrrole/riboflavin oxidase biomimetic recognition for direct and ultrasensitive electrochemical detection of prostate cancer biomarker. Sensors and Actuators B: Chemical, 138410.
[21] Fu, J., Liu, Y. (2023) Single-atom nanomaterials in electrochemical sensors applications. Chemosensors, 11(9), 486.
[22] Jimenez-Falcao, S., Méndez-Arriaga, J. M., Garcia-Almodovar, V., Garcia-Valdivia, A. A., Gomez-Ruiz, S. (2022) Gold nanozymes: smart hybrids with outstanding applications. Catalysts, 13(1), 13.
[23] Mao, L., Li, M., Li, Z., Feng, Y., Liu, L., Qin, R., Yuan, L. (2025) Development of a label-free electrochemical immunosensor based on self-assembled peptide-templated Au/Cu bimetallic nanozymes for sensitive detection of carcinoembryonic antigen (CEA). Microchimica Acta, 192(6), 375.
[24] Yang, F., Jiang, G., Yan, F., Chang, Q. (2019) Fe/C magnetic nanocubes with enhanced peroxidase mimetic activity for colorimetric determination of hydrogen peroxide and glucose. Microchimica Acta, 186(7), 417.
[25] Cheng, Y., Xia, Y. D., Sun, Y. Q., Wang, Y., Yin, X. B. (2024) “Three‐in‐one” nanozyme composite for augmented cascade catalytic tumor therapy. Advanced Materials, 36(8), 2308033.
[26] Park, J. Y., Jeon, J. H., Lim, H. R., Choa, Y. H. (2024) Wide detection range for non-enzymatic glucose monitoring by utilizing LDHs-coated CuO nanowires in biological media. Chemical Engineering Journal, 485, 149841.
[27] Wang, Z., Zhang, J., Jian, R., Liao, J., Xiong, X., Huang, K. (2020) Room temperature ultrafast synthesis of zinc oxide nanomaterials via hydride generation for non-enzymatic glucose detection. Microchemical Journal, 159, 105396.
[28] Zhu, Y., Wu, J., Han, L., Wang, X., Li, W., Guo, H., Wei, H. (2020) Nanozyme sensor arrays based on heteroatom-doped graphene for detecting pesticides. Analytical chemistry, 92(11), 7444-7452.
[29] Nataraj, N., Chen, T. W., Gan, Z. W., Chen, S. M., Hatshan, M. R., Ali, M. A. (2022) Bifunctional 3D-MOF-based nanoprobes for electrochemical sensing and nanozyme enhanced with peroxidase mimicking for colorimetric detection of acetaminophen. Materials Today Chemistry, 23, 100725.
[30] Li, Y., Zhou, H., Song, Q., Zou, M., Wei, Y., Zhang, Q. (2024) MnO2 nanoparticles as tandem nano-enzyme for colorimetric flexible sensor in sweat. Microchemical Journal, 204, 110973.
[31] Yuan, H., Hilal, M., Ali, Y., Abdo, H. S., Cai, Z., Kim, H., Ullah, U., Fayaz, H., Xie, W., Han, J. I. (2024) High-performance ZnO: CuO composite-based fiber-shaped electrode for non-enzymatic glucose sensing in biological fluids. Surfaces and Interfaces, 54, 105266.
[32] He, Q., Zhang, L. (2025) Bimetallic and multimetallic nanozymes: synergistic catalysis for advanced biomedical and health applications. RSC Advances, 15(55), 47648-47665.
[33] Song, G., Zheng, X., Zhang, Z., Fauconnier, M. L., Li, C., Chen, L., Zhang, D. (2025) Streamlined Synthesis of Ga‐Cu dual single-atom nanozymes for advanced electrochemical sensing applications. Advanced Functional Materials, 35(49), 10909.
[34] Zhuang, Z., Yu, Y., Dong, S., Sun, X., Mao, L. (2024) Carbon-based nanozymes: design, catalytic mechanisms, and environmental applications. Analytical and Bioanalytical Chemistry, 416(27), 5949-5964.
[35] Sun, H., Zhou, Y., Ren, J., Qu, X. (2018) Carbon nanozymes: enzymatic properties, catalytic mechanism, and applications. Angewandte Chemie International Edition, 57(30), 9224-9237.
[36] Zhong, J., Zhang, Y., Yan, C., Chen, J., Tang, X., Xu, P., Qiu, P. (2024) Rapid aqueous preparation of carbon quantum dots and their application as nanozyme for the detection of hydrogen peroxide and uric acid. Microchemical Journal, 201, 110529.
[37] Boruah, P. K., Borthakur, P., Neog, G., Le Ouay, B., Afzal, N. U., Manna, P., Das, M. R. (2023) Porous nitrogen-doped crumpled graphene nanoparticles: a metal-free nanozyme for selective detection of dopamine in aqueous medium and human serum. ACS Applied Nano Materials, 6(3), 1667-1677.
[38] Sun, W., Wang, N., Zhou, X., Sheng, Y., Su, X. (2022) Co, N co-doped porous carbon-based nanozyme as an oxidase mimic for fluorescence and colorimetric biosensing of butyrylcholinesterase activity. Microchimica Acta, 189(9), 363.
[39] Wang, Q., Yang, S., Zhang, L., Xiong, L., He, X., Chen, C., Dong, Y., Deng, G. (2026) Cu/N co-doped carbon dots nanozyme with enhanced peroxidase-like activity for dual-modal colorimetric-fluorescent uric acid detection. Microchemical Journal, 117264.
[40] Ye, H., Lai, Y., Wu, Z., Li, G., Hua, Q., Zhu, W. (2025) Carbon-based nanozymes: catalytic mechanisms, performance tuning, and environmental and biomedical applications. Analytical Methods, 17(31), 6264-6281.
[41] Dinu, L. A., Kurbanoglu, S. (2023) Enhancing electrochemical sensing through the use of functionalized graphene composites as nanozymes. Nanoscale, 15(41), 16514-16538.
[42] Yin, B., Jiang, Z., Muhammad, R., Liu, J., Wang, J. (2025) Nanozyme-Powered Multimodal Sensing for Pesticide Detection. Foods, 14(11), 1957.
[43] Zhong, W., Song, L., Wang, Y., Gu, Y., Miao, Y., An, Y. (2025) Erythrocyte-like BiVO4 and Bifunctional-Nanozyme based dual-signal Output Electrochemical Immunosensor: Enabling the precise and ultrasensitive detection of CA19-9. Talanta, 129070.
[44] Wang, W., Wu, Z., Shi, P., Wu, P., Qin, P., Yu, L. (2021) Antibacterial effect of chitosan-modified Fe3O4 nanozymes on Acinetobacter baumannii. Journal of Microbiology and Biotechnology, 32(2), 263.
[45] Xia, N., Gao, F., Zhang, J., Wang, J., Huang, Y. (2024) Overview on the development of electrochemical immunosensors by the signal amplification of enzyme-or nanozyme-based catalysis plus redox cycling. Molecules, 29(12), 2796.
[46] Niu, K., Chen, J., Lu, X. (2023) Versatile biomimetic catalyst functionalized nanozymes for electrochemical sensing. Chemical Engineering Journal, 475, 146491.
[47] Xue, S., Chen, G., Lai, Y., Zhang, R., Cheng, Y., Liu, J., Che, R. (2024) Nanozyme catalytic performance enhancement through defect and electronic structure regulation of metal-doped NiCo2O4@ Pd. Nano Letters, 24(31), 9591-9597.
[48] Miao, R., Zhang, Y., Sha, H., Ma, W., Huang, Y., Chen, H. (2025) Multifunctional carbon dot-based dual-channel and dual-signal sensors for ribonucleotide discrimination and Fe3+ detection. Journal of Materials Chemistry B, 13(22), 6444-6455.
[49] Jia, Z., You, Q., Wu, J., Wu, T., Yang, L., Li, L., Dong, W. F., Chang, Z. M. (2025) Fluorescence/colorimetric dual-mode probe based on nitrogen, sulfur, and boron Codoped MXene quantum dots for detection of glutathione and imaging in mitochondria. ACS Applied Materials & Interfaces, 17(37), 51754-51765.
[50] Bagherpour, S., Pérez-García, L. (2024) Recent advances on nanomaterial-based glutathione sensors. Journal of Materials Chemistry B, 12(34), 8285-8309.
[51] Xu, Z., Long, L. L., Chen, Y. Q., Chen, M. L., Cheng, Y. H. (2021) A nanozyme-linked immunosorbent assay based on Metal-Organic Frameworks (MOFs) for sensitive detection of aflatoxin B1. Food Chemistry, 338, 128039.
[52] Gao, S., Sun, Q., Katona, J., Zhang, D., Zhang, Y., Zou, X. (2025) Recent advances in Metal-Organic Framework nanozyme (MOFzyme)-based biosensors for detecting food contaminants. Journal of Agricultural and Food Chemistry, 73(37), 23045-23077.
[53] Sohrabi, H., Maleki, F., Khaaki, P., Kadhom, M., Kudaibergenov, N., Khataee, A. (2023) Electrochemical-based sensing platforms for detection of glucose and H2O2 by porous Metal-Organic Frameworks: a review of status and prospects. Biosensors, 13(3), 347.
[54] Hosseinzadeh, B., Rodriguez-Mendez, M. L. (2023) Electrochemical sensor for food monitoring using Metal-Organic Framework materials. Chemosensors, 11(7), 357.
[55] Leng, M., Cui, Y., Feng, Q., Dong, Z. Z., Miao, X. (2025) In situ monitoring of dynamic H2O2 produced upon the external stress of plants using Ag@ ZIF-67 based fluorescence sensor. Sensors and Actuators B: Chemical, 433, 137472.
[56] Ling, P., Cheng, S., Chen, N., Qian, C., Gao, F. (2020) Nanozyme-modified Metal-Organic Frameworks with multienzymes activity as biomimetic catalysts and electrocatalytic interfaces. ACS Applied Materials & Interfaces, 12(15), 17185-17192.
[57] Shi, Y., Zou, Y., Khan, M. S., Zhang, M., Yan, J., Zheng, X., Wang, W., Xie, Z. (2023) Metal-Organic Framework-derived photoelectro-chemical sensors: structural design and biosensing technology. Journal of Materials Chemistry C, 11(11), 3692-3709.
[58] Hou, C., Cheng, D., Zou, S., Gao, J., Wang, J., Wang, Y. (2023) Rational design of magnetic MOFs-COFs hybrid nanozyme for the colorimetric detection of phenol. Journal of Environmental Chemical Engineering, 11(5), 110914.
[59] Ai, Y., Shi, F., Han, X., Li, X., Fu, W., Wang, B., Zhang, X., Sun, W. (2023) Fe-doped two-dimensional graphite carbon nitride: An effective nanozymetic catalyst for electrochemical detection of cell-released H2O2. Microchemical Journal, 195, 109454.
[60] Gajare, B., Kanp, T., Aalhate, M., Dhuri, A., Manoharan, B., Rode, K., Nair, R., Paul, P., Singh, P. K. (2025) Emerging paradigms in nanozyme: strategies in fabrication, cancer targeting, and biosensing. ACS Applied Bio Materials, 8(8), 6588-6612.
[61] Wang, X., Wang, Y., Liu, Y., Cao, X., Zhang, F., Xia, J., Wang, Z. (2024) MOF-derived porous carbon nanozyme-based flexible electrochemical sensing system for in situ and real-time monitoring of H2O2 released from cells. Talanta, 266, 125132.
[62] Wu, Z., Sun, L. P., Zhou, Z., Li, Q., Huo, L. H., Zhao, H. (2018) Efficient nonenzymatic H2O2 biosensor based on ZIF-67 MOF derived Co nanoparticles embedded N-doped mesoporous carbon composites. Sensors and Actuators B: Chemical, 276, 142-149.
[63] Portorreal-Bottier, A., Gutierrez-Tarrino, S., Calvente, J. J., Andreu, R., Roldán, E., Oña-Burgos, P., Olloqui-Sariego, J. L. (2022) Enzyme-like activity of cobalt-MOF nanosheets for hydrogen peroxide electrochemical sensing. Sensors and Actuators B: Chemical, 368, 132129.
[64] Jouyban, A., Amini, R. (2021) Layered Double Hydroxides as an efficient nanozyme for analytical applications. Microchemical Journal, 164, 105970.
[65] Tian, L., Qi, J., Qian, K., Oderinde, O., Cai, Y., Yao, C., Song, W., Wang, Y. (2018) An ultrasensitive electrochemical cytosensor based on the magnetic field assisted binanozymes synergistic catalysis of Fe3O4 nanozyme and reduced graphene oxide/molybdenum disulfide nanozyme. Sensors and Actuators B: Chemical, 260, 676-684.
[66] Hu, Y., Hojamberdiev, M., Geng, D. (2021) Recent advances in enzyme-free electrochemical hydrogen peroxide sensors based on carbon hybrid nanocomposites. Journal of Materials Chemistry C, 9(22), 6970-6990.
[67] Liu, Y., Wang, X., Li, X., Qiao, S., Huang, G., Hermann, D. M., Doeppner, T. R., Zeng, M., Liu, W., Xu, G., Ren, L., Zhang, Y., Liu, W., Casals, E., Li, W., Wang, Y. C. (2021) A co-doped Fe3O4 nanozyme shows enhanced reactive oxygen and nitrogen species scavenging activity and ameliorates the deleterious effects of ischemic stroke. ACS Applied Materials & Interfaces, 13(39), 46213-46224.
[68] Alqarni, A. O., Alqahtani, R. A. A., Mahmoud, A. M., Almabadi, M. H., Alshareef, F. M., Goda, M. N., Ail, R., El-Wekil, M. M. (2026) Dual-modal colorimetric and fluorescent sensing of antineoplastic drug fludarabine via bimetallic-doped nitrogen carbon dots nanozyme. Journal of Cluster Science, 37(1), 6.
[69] Wang, S., Wang, L., Yan, X., Jiang, B. (2025) Cascade catalytic nanozymes: design, classification, and biomedical applications. ACS Applied Materials & Interfaces, 17(32), 45354-45381.
[70] Sherazee, M., Ahmed, S. R., Das, P., Srinivasan, S., Rajabzadeh, A. R. (2023) Electrochemically enhanced peroxidase-like activity of nanohybrids for rapid and sensitive detection of H2O2 and Dopamine. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 679, 132576.
[71] Puiu, M., Istrate, O. M., Mirceski, V., Bala, C. (2023) Ultrasensitive detection of hydrogen peroxide using methylene blue grafted on molecular wires as nanozyme with catalase-like activity. Analytical Chemistry, 95(44), 16185-16193.
[72] Shi, Z., Li, K., Li, J., Ding, Y., Zheng, X. (2023) Morphological dependency of antioxidant enzyme-like activities of nanoceria in energy-band structure aspect. Journal of Nanoparticle Research, 25(10), 200.
[73] Lu, N., Liu, Y., Yan, X., Xu, Z., Xing, Y., Song, Y., Zhao, P., Liu, M., Gu, Y., Zhang, Z., Zhai, S. (2022) Bioinspired surface modification of graphene-based hybrids as nanozyme sensors for simultaneous detection of dopamine and uric acid. ACS Applied Nano Materials, 5(8), 11361-11370.
[74] Ma, X., Zheng, H., Fang, L., Zhou, H., Shi, W., Zhao, W. (2025) Anchoring atomically distributed Mn with N, F Co-doped ultrathin carbon matrixes as multifunctional nanozymes to boost electrochemical sensing performance. Sensors and Actuators B: Chemical, 426, 137075.
[75] Luo, Q., Shao, N., Zhang, A. C., Chen, C. F., Wang, D., Luo, L. P., Xiao, Z. Y. (2023) Smart biomimetic nanozymes for precise molecular imaging: application and challenges. Pharmaceuticals, 16(2), 249.
[76] Xu, D., Wu, L., Yao, H., Zhao, L. (2022) Catalase-like nanozymes: classification, catalytic mechanisms, and their applications. Small, 18(37), 2203400.
[77] Wang, H., Liu, C., Feng, T., Zhang, Y., Wu, Y., Dai, Z., Yi, C. (2025) Enzyme-based electrochemical sensing systems for on-site detection: recent progress and prospects. Small, 21(47), e07926.
[78] Wang, X., Li, Q. Z., Zhao, Y., Gao, X. (2025) Recent advances in Oxidase-like nanozymes: mechanisms, prediction models, and applications. ACS Applied Materials & Interfaces, 17(49), 66110-66150.
[79] Chen, G. Y., Luo, M. L., Chen, L., Chai, T. Q., Wang, J. L., Chen, L. X., Yang, F. Q. (2023) Rapid and sensitive detection of alkaline phosphatase and glucose oxidase activity through fluorescence and colorimetric dual-mode analysis based on CuO NPs@ ZIF-8 mediated enzyme-cascade reactions. Nanoscale Advances, 5(18), 4950-4967.
[80] Sheng, J., Wu, Y., Ding, H., Feng, K., Shen, Y., Zhang, Y., Gu, N. (2024) Multienzyme‐like nanozymes: regulation, rational design, and application. Advanced Materials, 36(10), 2211210.
[81] Tang, J., Li, Y., Yao, Y., Hu, R. (2025) Colorimetric/electrochemical dual-mode aptasen-sor with phosphatase-like ceria nanozyme for fast and ultrasensitive detection of Ochratoxin A. Sensors and Actuators B: Chemical, 432, 137482.
[82] Xia, J., Li, Z., Ding, Y., Shah, L. A., Zhao, H., Ye, D., Zhang, J. (2024) Construction and application of nanozyme sensor arrays. Analytical Chemistry, 96(21), 8221-8233.
[83] Ahmed, S. R., Cirone, J., Chen, A. (2019) Fluorescent Fe3O4 quantum dots for H2O2 detection. ACS Applied Nano Materials, 2(4), 2076-2085.
[84] Stasyuk, N., Smutok, O., Demkiv, O., Prokopiv, T., Gayda, G., Nisnevitch, M., Gonchar, M. (2020) Synthesis, catalytic properties and application in biosensorics of nanozymes and electronanocatalysts: a review. Sensors, 20(16), 4509.
[85] Sindhu, R. K., Najda, A., Kaur, P., Shah, M., Singh, H., Kaur, P., Cavalu, S., Jaroszuk-Sierocińska, M., & Rahman, M. H. (2021) Potentiality of nanoenzymes for cancer treatment and other diseases: current status and future challenges. Materials, 14(20), 5965.
[86] Gao, S., Xu, X., Zheng, X., Zhang, Y., Zhang, X. (2025). Bimetallic gold-platinum (AuPt) nanozymes: recent advances in synthesis and applications for food safety monitoring. Foods, 14(18), 3229.
[87] Yang, R., Liu, Z., Chen, H., Zhang, X., Sun, Q., El-Mesery, H. S., Lu, W., Dai, X., Xu, R. (2025) Advances in nanozyme catalysis for food safety detection: a comprehensive review on progress and challenges. Foods, 14(15), 2580.
[88] Das, B., Franco, J. L., Logan, N., Balasubramanian, P., Kim, M. I., Cao, C. (2021) Nanozymes in point-of-care diagnosis: an emerging futuristic approach for biosensing. Nano-Micro Letters, 13(1), 193.
[89] Jeon, H. J., Kim, H. S., Chung, E., Lee, D. Y. (2022) Nanozyme-based colorimetric biosensor with a systemic quantification algorithm for noninvasive glucose monitoring. Theranostics, 12(14), 6308.
[90] Thamilselvan, A., Kim, M. I. (2024) Recent advances on nanozyme-based electrochemical biosensors for cancer biomarker detection. TrAC Trends in Analytical Chemistry, 177, 117815.
[91] Zhao, N., Shi, P., Wang, Z., Sun, Z., Sun, K., Ye, C., Fu, L., Lin, C. T. (2024) Advances in surface-enhanced raman spectroscopy for urinary metabolite analysis: Exploiting noble metal nanohybrids. Biosensors, 14(12), 564.
[92] Feng, J., Yao, S., Mei, X., Xie, J., Wang, X., Zhi, H., Shang, R., Yang, Y., Hu, L., Yan, Z. (2025) Au-ag quantum dot nanozyme identified for multi-mode fluorescent, UV-vis and intelligent RGB monitoring and removal of toxic Hg2+ and S2− in food samples. Food Chemistry, 483, 144308.
[93] Chen, T., Ge, Y., Lu, X., Hu, J., Karimi-Maleh, H., Wen, Y., Wang, X., Huang, Z., Li, M. (2024) Ultrasound-electrochemistry assisted liquid-phase co-exfoliation of phosphorene decorated by Au-Ag bimetallic nanoparticles as nanozyme for smartphone-based portable sensing of 4-nitrophenol. Microchimica Acta, 191(8), 446.
[94] Cen, M., Shen, H., Li, X., Yu, S. (2026) A target-triggered cascade colorimetric biosensor based on PCN-222 (Fe)/GOx nanozyme for rapid detection of antibiotic resistance genes. Biosensors and Bioelectronics, 304, 118663.
[95] Öğüt, E., Kip, Ç., Gökçal, B., Tuncel, A. (2019) Aggregation-resistant nanozyme containing accessible magnetite nanoparticles immobilized in monodisperse-porous silica microspheres for colorimetric assay of human genomic DNA. Journal of Colloid and Interface Science, 550, 90-98.
[96] Sridara, T., Upan, J., Saianand, G., Tuantranont, A., Karuwan, C., Jakmunee, J. (2020) Non-enzymatic amperometric glucose sensor based on carbon nanodots and copper oxide nanocomposites electrode. Sensors, 20(3), 808.
[97] Wang, M., Zhu, P., Liu, S., Chen, Y., Liang, D., Liu, Y., Chen, W., Du, L., Wu, C. (2023) Application of nanozymes in environmental monitoring, management, and protection. Biosensors, 13(3), 314.
[98] Wong, E. L., Vuong, K. Q., Chow, E. (2021) Nanozymes for environmental pollutant monitoring and remediation. Sensors, 21(2), 408.
[99] Li, X., Liu, J., Chen, J., Qiu, H., Niu, X. (2023) Recent progress in nanozyme-based sensors for ion detection: strategies, trends, and challenges. Sensors & Diagnostics, 2(2), 307-319.
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Xiao, J., Duan, Y., Zhu, H., Ling, K., Wang, G., Li, Y., Jiang, Q., Wang, Z., Zou, S. (2026) Research Progress on Nanozyme-based Electrochemical Sensors. Scientific Research Bulletin, 3(1), 58-76. https://doi.org/10.71052/srb2024/LBFH4830